By M. Dean Savage (auth.), Dr. Thomas Meier, Dr. Falk Fahrenholz (eds.)
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Extra resources for A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis
0CC/HONS" 2. SI 3. 20% pipuridinl;! 2 Synthesis of PHIBANA-CCK-Bs (7) 39 Christoph Thiele and Falk Fahrenholz Binding to anti-CCK antisera and CCK-B receptors Binding of eH]BANA-CCK-Ss to anti-CCK antisera occurred in a specific, saturable manner with high affinity. 3 nM). 1 nM) and low nonspecific binding to digitonin-solubilized pig brain cortical membranes. To examine detrimental effects of photoelution toward proteins, we determined the binding capacity of CCK-B receptor preparations that had been irradiated under the same conditions as used for photoelution.
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03 mmol) of 2,4,5-trichlorophenol was dissolved in 40 ml of ethyl acetate. 04 mmol) of N,N'-dicyclohexyl carbodiimide (DCC) in 10 ml of ethyl acetate. After stirring for 16 hours, the white precipitate was removed by filtration, and the solvent was evaporated. The residue was recrystallized from CH2C12/pentane to give a slightly yellow product. 65 mmol, 81 %) . Melting point 124--125 0c. 0 min. 88 (s, 2H, benzyl-H). Positive ion electron ionization mass spectroscopy (EI -MS): mlz = 437 1M + Hr.