Advances in Biochemical Engineering, Volume 009: Microbial by T.K. Ghose ,A. Fiechter, N.Blakebrough

By T.K. Ghose ,A. Fiechter, N.Blakebrough

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It was shown that there were two peculiarities important for the interaction between the cell surface and the hydrocarbon: 1) porous structure of the surface layer and 2) formation of a mannan-fatty acid-complex during growth on hydrocarbons thereby increasing the lipophility of the surface. The formation of the mannan-fatty acid-complex was studied during a substrate shift from glucose to hydrocarbons. The decrease of affinity on glucose is considered to be a consequence of a diminished lipophility at the cell surface.

10 shows the rate for C. tropicalis. $ CELL CONC. [ g / l ) Fig. 9. Oxygen uptake rate on drop-form n-tetradecane for alanine-gzownC. 6 CELL COliC. =) Fig. 10. Oxygen uptake rate on drop-form n-tetradecane for alanine-grown C. tropicalis I81 n-tetradecane concentration: 10s ppm for C. intermedia Qo~ (DH)max = 5 x 10 -3 (mole 02)- (g cell) - ] • h -1 and for C. 3 x 10 - 3 (mole 02). (g cell) - l • h - l • Qo2 (DH)max was not equal to Qo, (AH)max for C. tropicalis. tetradecane is not formed during the measurement of the oxygen uptake rate on drop-form n-tetradeeane.

Sci, 45 (1958). 7. : Die Branntweinwirtschaft 100, 23,567 (1960). 8. lyerusalismky, N. : Meted protochnogo kuitivirovaniya microorganismov. Moscwa; Pishcepromisdat, 9 (1960). 9. Ierusalimsky, N. : Microbiologia 30° 5 , 8 1 8 - 8 2 5 (1961). 10. Iyerusalimski, N. : Upravlyaemii biosyntes. Moscwa; Nauka, 5,398 (1966). 11. Kretovich, V. /Yarovenko, V. : Fermentnii preparaty v pishchevoi promyshlennosti. Moscwa; Pishchevaya promyshlennost, 536 (1975). 12. : Nepretyynoye kultivirovanie mikroorganismov.

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